Adenovirus (ADV) is a non-enveloped, linear, double-stranded DNA virus with broad-spectrum cellular and tissue infectivity. Its infection process is vigorous, making it suitable for experiments involving high gene expression in a short time. Compared to other viruses, adenovirus has the advantage of longer insertion fragments and stronger expression activity. In vitro, adenovirus vectors exhibit high transduction efficiency (nearly 100%) and can transduce different cell types, making it an excellent tool for gene delivery to large numbers of difficult-to-transfect cells. However, in vivo, due to the strong immunogenicity and vigorous infection of adenovirus, it often induces local tissue inflammation and immune responses in animals, affecting the objectivity of animal signs and experimental results.